ABSTRACT
INTRODUCTION: Dengue is the most prevalent arboviral disease in tropical areas. In Mato Grosso, outbreaks are reported every year, but studies on dengue in this state are scarce. METHODS: Natural transovarial infection of Aedes aegypti by a flavivirus was investigated in the Jardim Industriário neighborhood of Cuiabá, Mato Grosso. Eggs were collected with ovitraps during the dry, intermediate, and rainy seasons of 2012. After the eggs hatched and the larvae developed to adulthood, mosquitoes (n = 758) were identified and allocated to pools of 1-10 specimens according to the collection location, sex, and climatic period. After RNA extraction, multiplex semi-nested RT-PCR was performed to detect the four dengue virus (DENV) serotypes, yellow fever virus, West Nile virus and Saint Louis encephalitis virus. RESULTS: DENV-4 was the only flavivirus detected, and it was found in 8/50 pools (16.0%). Three of the positive pools contained females, and five contained males. Their nucleotide sequences presented 96-100% similarity with DENV-4 genotype II strains from Manaus, Amazonas. The minimum infection rate was 10.5 per 1000 specimens, and the maximum likelihood estimator of the infection rate was 11.6 (95% confidence interval: 4.8; 23.3). CONCLUSIONS: This study provides the first evidence of natural transovarial infection by DENV-4 in Ae. Aegypti in Mato Grosso, suggesting that this type of infection might serve as a mechanism of virus maintenance during interepidemic periods in Cuiabá, a city where dengue epidemics are reported every year. These results emphasize the need for efficient vector population control measures to prevent arbovirus outbreaks in the state. .
Subject(s)
Animals , Humans , Mice , Kinesins/metabolism , Protein Biosynthesis , Cell Line , Centrifugation, Density Gradient , Gene Knockdown Techniques , Immunoprecipitation , Interphase , Kinesins/antagonists & inhibitors , Kinesins/genetics , Microtubules/metabolism , Peptide Chain Initiation, Translational , Protein Binding , Pyrimidines/pharmacology , RNA Interference , Ribosomes/metabolism , Thiones/pharmacologyABSTRACT
Objetivo: avaliar a implementação da assistência pré-natal em unidades de Saúde da Família (SF) no município de Cuiabá-MT, Brasil. Métodos: estudo de avaliação normativa, com estratégia de pesquisa de estudo de caso realizado em duas unidades de SF caso I e caso II ; adotou-se a dimensão de conformidade do pré-natal ao Programa de Humanização no Pré-natal e Nascimento (PHPN); utilizaram-se parâmetros para classificação do grau de implementação implementado (≥80 por cento), parcialmente implementado (40 a 79,9 por cento) e implementação crítica (<40 por cento). Resultados: participaram do estudo 69 gestantes; 19/30 (caso I) e 17/39 (caso II) tiveram seis ou mais consultas de pré-natal; 27/30 (caso I) e 10/39 (caso II) tiveram VDRL realizado na 1ª consulta; os casos I e II apresentaram, respectivamente, 67,9 por cento e 64,3 por cento de implementação no elemento insumo, e 69,7 por cento e 70,0 por cento no elemento atividade. Conclusão: a assistência pré-natal apresentou implementação parcial nos dois casos, sinalizando necessidade de melhorar sua qualidade.
Objective: to evaluate antenatal care implementation in Family Health (FH) facilities in Cuiabá/MT. Methods: normative evaluation study using two FH centres as case studies (Case I and Case II). The extent of antenatal compliance was evaluated according to Antenatal and Birth Humanization Program standards. Degree of implementation was classified using the following parameters: Implemented: ≥80%; Partially implemented: 40 per cent-79.9 per cent; Implementation Critical: <40 per cent. Differences between cases were checked using Fishers exact test. Results: 69 pregnant women were recruited. 19/30 (Case I) and 17/39 (Case II) had six or more antenatal consultations. 27/30 (Case I) and 10/39 (Case II) had VDRL screening at the 1st consultation. Case I had 67.9 per cent input implementation and 69.7 per cent activity implementation. Case II had 64.3 per cent and 70 per cent, respectively. Conclusion: antenatal care was partially implemented in both cases, indicating the need to adopt measures to improve its quality.
Subject(s)
Primary Health Care/organization & administration , Health Evaluation/statistics & numerical data , Prenatal Care , Case ReportsABSTRACT
Este estudo tem como objetivo analisar a vigilância epidemiológica no Plano de Intensificação das Ações de Controle de Malária (PIACM), nos dezoito municípios mato-grossenses, de 1999 a 2002. Realizou-se uma pesquisa avaliativa do tipo análise de implantação. A análise foi realizada através do Modelo Lógico de Avaliação, considerando quatro dimensões de análise - contexto externo e político, contexto organizacional, implementação e efetividade - e cinco bases de evidências, sendo três de dados primários e os outros secundários. Os municípios foram denominados de CASOS. Os dados foram coletados por questionários semi-estruturados. A implementação das ações do PIACM apresentou-se adequada apenas em 5,6 por cento dos CASOS. A efetividade das ações em apenas um CASO e nenhum CASO teve condição adequada nas características contextuais. Os fatores que mais influenciaram a implementação foram: rotatividade dos gestores municipais e profissionais de saúde; capacitação fragmentada e esporádica; promoção e prevenção à saúde limitada aos profissionais da atenção básica. Conclui-se que a vigilância epidemiológica, no PIACM, mostrou-se desarticulada, com problemas de ordem estrutural, o que vem dificultando o pleno desenvolvimento das ações.
The objective of this study is to analyze the epidemiologic surveillance in the Malaria Control Actions Intensification Plan (PIACM) in the 18 cities of the state of Mato Grosso, from 1999 to 2002. An evaluative research of the type implantation analysis was conducted. The analysis was carried out using a logic model considering 4 dimensions - external and political context, organizational context, implementation and effectiveness - and 5 evidence bases, 3 of primary data and 2 of secondary data. The cities are called CASE. The data were collected using semi-structured questionnaires. The implementation of the PIACM actions was found adequate in only 5,6 percent of CASES. The effectiveness of the actions was adequate in only one CASE and none of the CASES showed adequate contextual characteristics. The principal factors affecting the implementation were: frequent changes of the local health managers and professionals, fragmented and sporadic training and health promotion and prevention actions limited to the primary care team. In conclusion, structural problems and lack of articulation of epidemiologic surveillance hamper the full development of the PIACM actions.